芦荟素/抗腺苷A2B受体（细胞外）-FITC抗体/AAR-003-F/2 x 50µl-免疫在线蚂蚁淘旗下平台

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商品详细芦荟素/抗腺苷A2B受体（细胞外）-FITC抗体/AAR-003-F/2 x 50µl

芦荟素/抗腺苷A2B受体（细胞外）-FITC抗体/AAR-003-F/2 x 50µl

商品编号： AAR-003-F-50µl

品牌： Alomone Labs

市场价： ¥10540.00

美元价： 6324.00

产地：美国(厂家直采)

公司：

产品分类：受体

公司分类： Receptors

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

立即询价

商品介绍

Cat#:AAR-003-F

LyophilizedPowder

AntigenIncl.

ShippedatRoomTemp.

Type:Polyclonal

Source:Rabbit

Reactivity:h,m

Mayalsoworkin:r

Immunogen

PeptideKDSATNN(S)TEPWDGTTNESC,correspondingtoaminoacidresidues147-166ofhuman A_2BAR(Accession P29275).2^nd extracellularloop.

Anti-Adenosine A2B Receptor (extracellular)-FITC Antibody -

Accession(Uniprot)NumberP29275

GeneID136

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyRat -14/19aminoacidresiduesidentical;mouse-13/19aminoacidresiduesidentical

PurityAffinitypurifiedonimmobilizedantigen.

FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN₃.

LabelFluoresceinisothiocyanate(FITC).

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution50µldoubledistilledwater(DDW).

Antibodyconcentrationafterreconstitution1mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).

ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.

Controlantigenreconstitution100µldoubledistilledwater(DDW).

Controlantigenstorageafterreconstitution-20°C.

PreadsorptionControl1µgpeptideper1µgantibody.

StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#AAR-003),anddirectflowcytometry(labeledantibody).

Applications:fc

Mayalsoworkin:ic,lci

Directflowcytometry

DirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:

^___Cellsalone.
^___Cells+RabbitIgGisotypecontrol-FITC.
^___Cells+Adenosine A_2B Receptor (extracellular)-FITCAntibody(AAR-003-F),5µg.

DirectflowcytometryofliveintactmouseJ774macrophagecells:

^___Cellsalone.
^___Cells+RabbitIgGisotypecontrol-FITC.
^___Cells+Adenosine A_2B Receptor (extracellular)-FITCAntibody(AAR-003-F),5µg.

References

Peeters,M.C.etal.(2011)FASEBJ.25,632.
Sun,Y.andHuang,P.(2016)Front.Chem.4,37.

Scientificbackground

ExtracellularadenosineisaubiquitoussignalingmoleculethatmodulatesawidearrayofBIOLOGicalprocesses.Therearecurrentlyfourknownsub-typesofadenosinereceptors:A₁,A_2A,A_2B andA₃.

TheadenosineA_2B receptorisatypicalclassA,Gprotein-coupledreceptorwithseventransmembranesegments,anextracellularN-terminusandanintracellularC-terminus.AdenosineA_2BRcausesanincreaseinintracellularcAMPlevelsbycouplingmainlytoG_s proteins,resultingintheactivationofadenylylcyclase¹ andsubsequentactivationofPKAandothercAMPeffectorssuchasEpac.Inaddition,theA_2BRcouplestoG_q andactivatesthePLCpathwaythatmediatesseveralcrucialfunctionsofA_2BR.A_2BRalsocouplestotheMAPKandarachidonicacidsignalingpathwaysandregulatesmembraneionchannelsprobablythroughG-proteinβγsubunits.

MultiplemoleculesthatbindtoA_2BRhavebeenidentifiedinanefforttounderstandthereceptor’sfunctionandregulation.NFκB1/p105isamemberoftheNFκBfamilyofproteinsthatperformregulatoryfunctionsindiversebiologicalprocessessuchasinflammationandcellsurvivalanddifferentiation,aswellasinvariousdiseases.Interestingly,AlthoughA_2BRactivationbyadenosineproducespro-inflammatoryeffectsthereceptoralsobindstospecificsitesonp105,preventsthepoly-ubiquitinationanddegradationofthep105proteinandinhibitsNFκBactivationthusreducinginflammation.

A_2BRishighlyexpressedinvarioustypesoftumorcellsortissuesandpromotestumor-cellproliferation.A_2BRwasfoundtobeoverexpressedincolorectalcarcinomacellsandtissues,andinhibitionofA_2BRblockstheproliferationofcoloncancercells.A_2BRhasbeenimplicatedinthepathophysiologyofvasculardiseases,renaldiseaseanddiabetes².

LyophilizedPowderControlAntigenIncluded

Lastupdate:24/03/2019

Anti-AdenosineA_2B Receptor(extracellular)Antibody(#AAR-003)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthehumanprotein.Theantibodycanbeusedinwesternblot,immunohistochemistry,livecellimaging,andindirectflowcytometryapplications.IthasbeendesignedtorecognizeA_2BARfromhuman,rat,andmousesamples.

Anti-Adenosine A_2B Receptor(extracellular)-FITCAntibody(#AAR-003-F)isdirectlyconjugatedtofluoresceinisothiocyanate(FITC).Thislabeledantibodycanbeusedinimmunofluorescentapplicationssuchasdirectflowcytometryusinglivecells.

Forresearchpurposesonly,notforhumanuse

Directflowcytometry

DirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:

^___Cellsalone.
^___Cells+RabbitIgGisotypecontrol-FITC.
^___Cells+Adenosine A_2B Receptor (extracellular)-FITCAntibody(AAR-003-F),5µg.

DirectflowcytometryofliveintactmouseJ774macrophagecells:

^___Cellsalone.
^___Cells+RabbitIgGisotypecontrol-FITC.
^___Cells+Adenosine A_2B Receptor (extracellular)-FITCAntibody(AAR-003-F),5µg.

References

Peeters,M.C.etal.(2011)FASEBJ.25,632.
Sun,Y.andHuang,P.(2016)Front.Chem.4,37.

品牌介绍

Alomone Labs是您离子通道研究中的伙伴，是离子通道、G蛋白耦联受体和神经信号工具的主要供应商。我们专业生产基础科学研究中所需的一抗、小分子、蛋白质和多肽。Alomone Labs提供的如下生命科学领域研究方案享誉海内外：离子通道研究领域（一系列全面的离子通道研究产品线，包括标记和未标记的抗体及其相关调节因子）、G蛋白耦联受体研究领域（提供了一整套不断增长的GPCR抗体、受体激动剂和拮抗剂产品名单）、神经信号通路研究（包括一些用于神经营养因子研究的最重要的抗体和生长因子）。 Alomone成立于1989年,已经成为全世界科学家信赖的最全面的离子通道研究工具供应商。所有Alomone产品均进行了生物实验验证，以冻干粉形式提供，可确保产品生物活性和保质期。Alomone可提供多种包装规格，灵活方便。作为Alomone Labs在中国的区域总代理，蚂蚁淘科技有限公司将为中国客户提供最全面的Alomone Labs产品以及客户订制化服务。

公司介绍

品牌分类

腺苷受体附着力等级GPCR 酸敏G蛋白偶联受体肾上腺素能受体

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