阿洛蒙/抗-α；1A肾上腺素能受体（细胞外）抗体/AAR-015/0.2 ml-免疫在线蚂蚁淘旗下平台

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商品详细阿洛蒙/抗-α；1A肾上腺素能受体（细胞外）抗体/AAR-015/0.2 ml

阿洛蒙/抗-α；1A肾上腺素能受体（细胞外）抗体/AAR-015/0.2 ml

商品编号： AAR-015-0.2ml

品牌： Alomone Labs

市场价： ¥9500.00

美元价： 5700.00

产地：美国(厂家直采)

公司：

产品分类：受体

公司分类： Receptors

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Cat#:AAR-015

LyophilizedPowder

AntigenIncl.

ShippedatRoomTemp.

Type:Polyclonal

Source:Rabbit

Reactivity:h,r

Mayalsoworkin:m

Immunogen

PeptideEDETI*SQINEEPG(C),correspondingtoaminoacidresidues171-183ofhumanα_1A-adrenoceptorwithreplacementofcysteine176(C₁₇₆)withserine(*S) (AccessionP35348).2^ndextracellularloop.

Anti-α1A-Adrenergic Receptor (extracellular) Antibody -

Accession(Uniprot)NumberP35348

GeneID148

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyHuman,rat,mouse,bovine-12/13aminoacidresiduesidentical.

PurityAffinitypurifiedonimmobilizedantigen.

FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN₃.

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.

Antibodyconcentrationafterreconstitution0.70mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).

ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.

Controlantigenreconstitution100µldoubledistilledwater(DDW).

Controlantigenstorageafterreconstitution-20°C.

PreadsorptionControl2µgpeptideper1µgantibody.

StandardqualitycontrolofeachlotWesternblotanalysis.

Applications:ih,wb

Mayalsoworkin:ifc,ip,lci

Westernblot

WesternblotanalysisofPC3celllinelysate(lanes1and3)andratbrainmembrane(lanes2and4):

1,2.Anti-α_1A-AdrenergicReceptor(extracellular)Antibody(#AAR-015),(1:200).
3,4.Anti-α_1A-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

Immunohistochemistry

Expressionofα_1A-Adrenergicreceptorinratcerebellum

ImmunohistochemicalstainingofratcerebellumusingAnti-α_1A-AdrenergicReceptor(extracellular)Antibody(#AAR-015),(1:100).A.α_1A-adrenoceptor(green)appearsinfibersofBergmannglia(trianglepointsatanexample).B.S100β(red),aMarkerofBergmannglia,isstainedinthesamesection.C.Mergeoftheimagesdemonstratesexpressionofα_1A-adrenoceptorinfibersofBergmannglia.α_1A-adrenoceptorisalsoexpressedincellsinthemolecularlayerthatares100β negative(arrowpointsatanexample).DAPIisusedasthecounterstain(blue).

References

IUPHARRECEPTORDATABASE|ADRENOCEPTORS.
Piascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.
Perez,D.M.etal.(1993)MolPharmacol44,784.
Minneman,K.P.(1988)Pharmacol.Rev.40,87.

Scientificbackground

Adrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions.

AdrenergicreceptorsaremembersoftheG-proteincoupledreceptor(GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,andacytoplasmiccarboxylterminus.

Adrenoceptorsaredividedintothreetypes:α₁,α₂ andβadrenoceptors. Eachtypeisfurtherdividedintoatleastthreesubtypes:α_1A,α_1B,α_1D,α_2A,α_2B,α_2C,β₁,β₂,β₃.^1,2Theyareexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem.^1,2

Allα₁-adrenoceptors(α₁-ARs)activatephospholipasesCandA2.³ Inadditiontomobilizingintracellularcalcium,theα₁-ARshavealsobeenshowntoactivatecalciuminfluxviavoltage-dependentand-independentcalciumchannels.⁴

Theα_1A-ARshaveatleastnineknownalternativesplicedisoforms.Theyarethemostabundantadrenergicreceptorinthebrain,andareconsideredpostsynapticreceptors.α_1A-ARsarealsoexpressedintheheart,liverandprostate.

LyophilizedPowderControlAntigenIncluded

Clickheretoreceivea25µlfreetrialsample!

Lastupdate:05/11/2018

AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanextracellularepitopeofthehumanα_1A-adrenergicreceptor.Anti-α_1A-AdrenergicReceptor(extracellular)Antibody(#AAR-015)canbeusedinwesternblotandimmunohistochemistryapplications.Ithasbeendesignedtorecognizeα_1A-adrenoceptorfromhuman,rat,andmousesamples.

Forresearchpurposesonly,notforhumanuse

Westernblot

WesternblotanalysisofPC3celllinelysate(lanes1and3)andratbrainmembrane(lanes2and4):

1,2.Anti-α_1A-AdrenergicReceptor(extracellular)Antibody(#AAR-015),(1:200).
3,4.Anti-α_1A-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

品牌介绍

Alomone Labs是您离子通道研究中的伙伴，是离子通道、G蛋白耦联受体和神经信号工具的主要供应商。我们专业生产基础科学研究中所需的一抗、小分子、蛋白质和多肽。Alomone Labs提供的如下生命科学领域研究方案享誉海内外：离子通道研究领域（一系列全面的离子通道研究产品线，包括标记和未标记的抗体及其相关调节因子）、G蛋白耦联受体研究领域（提供了一整套不断增长的GPCR抗体、受体激动剂和拮抗剂产品名单）、神经信号通路研究（包括一些用于神经营养因子研究的最重要的抗体和生长因子）。 Alomone成立于1989年,已经成为全世界科学家信赖的最全面的离子通道研究工具供应商。所有Alomone产品均进行了生物实验验证，以冻干粉形式提供，可确保产品生物活性和保质期。Alomone可提供多种包装规格，灵活方便。作为Alomone Labs在中国的区域总代理，蚂蚁淘科技有限公司将为中国客户提供最全面的Alomone Labs产品以及客户订制化服务。

公司介绍

品牌分类

腺苷受体附着力等级GPCR 酸敏G蛋白偶联受体肾上腺素能受体

联络我们

服务热线：4000-520-616

（限工作日9:00-18:00）

QQ ：1570468124

手机：18915418616

官网：http://www.alomone.com/

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