LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
ShippedatRoomTemp.ShippedatRoomTemp.
Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.
Type:Polyclonal
Source:Rabbit
Reactivity:h,m,r
ImmunogenPeptide(C)HRV(S)FEHYPIQAWQR,correspondingtoaminoacidresidues169-183ofmouseGPR68(Accession Q8BFQ3).2nd extracellularloop.
Accession(Uniprot)NumberQ8BFQ3
GeneID238377
PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
HomologyRat,mouse,human–14/15aminoacidresiduesidentical.
PurityAffinitypurifiedonimmobilizedantigen.
FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
Antibodyconcentrationafterreconstitution0.8mg/ml.
StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Controlantigenreconstitution100µldoubledistilledwater(DDW).
Controlantigenstorageafterreconstitution-20°C.
PreadsorptionControl1µgpeptideper1µgantibody.
StandardqualitycontrolofeachlotWesternblotanalysis.
Applications:ifc,wb
Mayalsoworkin:ic,ih,ip,lci
WesternblotWesternblotanalysisofratkidneylysates(lanes1and3)andmousekidneymembranes(lanes2and4):
1,2. Anti-GPR68(OGR1)(extracellular)Antibody (#AGR-042),(1:200).
3,4.Anti-GPR68(OGR1)(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
Westernblotanalysisofratheartmembranes:
1. Anti-GPR68(OGR1)(extracellular)Antibody (#AGR-042),(1:200).
2.Anti-GPR68(OGR1)(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
IndirectflowcytometryIndirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:
___Cells.
___Cells+goat-anti-rabbit-FITC.
___Cells+Anti-GPR68(OGR1)(extracellular)Antibody (#AGR-042),(2.5µg)+goat-anti-rabbit-FITC.
ReferencesThompson,M.D.etal.(2005)Crit.Rev.Clin.LabSci.42,311.
Xu,Y.andCasey,G.(1996)Genomics.35,397.
Zaslavsky,A.etal.(2006)Biochim.Biophys.Acta.1761,1200.
Mohebbi,N.etal.(2012)Cell.Physiol.Biochem.29,313.
ScientificbackgroundAllGPCRs,includingGPR68,aresinglepeptidespossessingseventransmembranedomainswithanextracellularN-terminusandanintracellularC-terminus1.GPR68isexpressedinseveraltissuesincludingthespleen,testis,smallbowel,brain,heart,lungandkidneys.Itsharesstronghomology(49-54%)withthehumanreceptor GPR42.
GPR68isprotonsensitiveinthepHrangeof6.8-7.8andactsasaprotonactivatedreceptor.GPR68stimulatesinositoltriphosphate(IP3)formationtherebyreleasingintracellularcalciumandactivatingthemitogenactivatedproteinkinase/extracellularsignal-regulated(ERK)kinasecascade.Inaddition,GPR68receptorregulatesNHE3andH+-ATPase,inresponsetoextracellularpHchangesinculturedHEK293renalcells.Thisactivationrequiresaclusterofhistidineresiduesintheextracellulardomainsofthereceptorandprotein-kinaseC(PKC)activation4.
LyophilizedPowderControlAntigenIncluded
Clickheretoreceivea25µlfreetrialsample!
Lastupdate:14/03/2019
Anti-GPR68(OGR1)(extracellular)Antibody(#AGR-042)isahighlyspecificantibodydirectedagainstanepitopeofthemouseovariancancerG-proteincoupledreceptor1.Theantibodycanbeusedinwesternblotandlivecellflowcytometryapplications.IthasbeendesignedtorecognizeGPR68fromrat,mouse,andhumansamples.
Forresearchpurposesonly,notforhumanuse
WesternblotWesternblotanalysisofratkidneylysates(lanes1and3)andmousekidneymembranes(lanes2and4):
1,2. Anti-GPR68(OGR1)(extracellular)Antibody (#AGR-042),(1:200).
3,4.Anti-GPR68(OGR1)(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
Westernblotanalysisofratheartmembranes:
1. Anti-GPR68(OGR1)(extracellular)Antibody (#AGR-042),(1:200).
2.Anti-GPR68(OGR1)(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
