阿洛蒙/抗-β；2-肾上腺素能受体（细胞外）抗体/AAR-016/50µl-免疫在线蚂蚁淘旗下平台

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商品详细阿洛蒙/抗-β；2-肾上腺素能受体（细胞外）抗体/AAR-016/50µl

阿洛蒙/抗-β；2-肾上腺素能受体（细胞外）抗体/AAR-016/50µl

商品编号： AAR-016-50µl

品牌： Alomone Labs

市场价： ¥7340.00

美元价： 4404.00

产地：美国(厂家直采)

公司：

产品分类：受体

公司分类： Receptors

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

立即询价

商品介绍

Cat#:AAR-016

LyophilizedPowder

AntigenIncl.

ShippedatRoomTemp.

Type:Polyclonal

Source:Rabbit

Reactivity:m,r

Mayalsoworkin:h

Immunogen

Peptide(C)NGSRAPDHDVTQERDE,correspondingtoaminoacidresidues15-30ofmouseADRB2 (AccessionP18762).Extracellular,N-terminus.

Anti-β2-Adrenergic Receptor (extracellular) Antibody -

Accession(Uniprot)NumberP18762

GeneID11555

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyHuman-15/16aminoacidresiduesidentical;rat-14/16aminoacidresiduesidentical.

PurityAffinitypurifiedonimmobilizedantigen.

FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN₃.

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution25µl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.

Antibodyconcentrationafterreconstitution0.7mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).

ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.

Controlantigenreconstitution100µldoubledistilledwater(DDW).

Controlantigenstorageafterreconstitution-20°C.

PreadsorptionControl1μgpeptideper1μgantibody.

StandardqualitycontrolofeachlotWesternblotanalysis.

Applications:ih,wb

Mayalsoworkin:ic,ifc,ip,lci

Westernblot

Westernblotanalysisofratheart(lanes1and3)andlung(lanes2and4)lysates:

1,3.Anti-β₂-AdrenergicReceptor(extracellular) Antibody(#AAR-016),(1:200).
2,4.Anti-β₂-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

Immunohistochemistry

Anti-β2-Adrenergic Receptor (extracellular) Antibody - Expression of β2-Adrenoceptor in rat lung

Expressionof β₂-Adrenoceptorinratlung

Immunohistochemicalstainingofparaffin-embeddedsectionofratlungusingAnti-β₂-AdrenergicReceptor(extracellular) Antibody(#AAR-016),(1:100).β₂-Adrenoceptorisexpressedintherespiratoryepithelium.Hematoxilinisusedasthecounterstain.

References

IUPHARRECEPTORDATABASE|ADRENOCEPTORS
Piascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.
Pullar,C.E.etal.(2006)FASEBJ.20,76.
Oostendorp,J.etal(2005)Am.J.Respir.Crit.CareMed.172,322.
Giembycz,M.A.andNewton,R.(2006)Eur.Respir.J.27,1286.

Scientificbackground

Adrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions.

AdrenergicreceptorsaremembersoftheG-proteincoupledreceptor(GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,andacytoplasmiccarboxylterminus.

Adrenoceptorsaredividedintothreetypes:α₁,α₂ andβ-adrenoceptors.Eachtypeisfurtherdividedintoatleastthreesubtypes:α_1A,α_1B,α_1D,α_2A,α_2B,α_2C,β₁,β₂,β₃.^1,2 Adrenoceptors areexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem.^1,2

β₂-Adrenoceptorsaremainlypostsynapticreceptors.Theyareexpressedonanumberoftissuesincludingbloodvessels,bronchi,gastrointestinaltract(GIT),skeletalmuscle,liverandmastcell.³ Functionalβ₂-adrenoceptorswerealsofoundonlymphocytes,macrophagesandneutrophils.^4,5 Theyarealsotheonlyβ-adrenoceptorsubtypeexpressedinkeratinocytes,fibroblastsandmelanocytes.³ Activationofβ₂-adrenoceptorsresultsinvasodilation,relaxationoftheGIT,inhibitionofhistaminereleasefrommastcellsandbronchodilation.SelectiveagoNISTsofβ₂-adrenoceptorsareusedtotreatasthmaandotherrelatedbronchospasticconditionssuchasChronicobstructivepulmonarydisease(COPD).⁵

LyophilizedPowderControlAntigenIncluded

Clickheretoreceivea25µlfreetrialsample!

Lastupdate:01/01/2019

Anti-β₂-AdrenergicReceptor(extracellular)Antibody(#AAR-016)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthemouseβ₂-adrenoceptor.Theantibodycanbeusedinwesternblotandimmunohistochemistryapplications.Ithasbeendesignedtorecognizeβ2ARfrommouse,rat,andhumansamples.

Forresearchpurposesonly,notforhumanuse

Westernblot

Westernblotanalysisofratheart(lanes1and3)andlung(lanes2and4)lysates:

1,3.Anti-β₂-AdrenergicReceptor(extracellular) Antibody(#AAR-016),(1:200).
2,4.Anti-β₂-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

品牌介绍

Alomone Labs是您离子通道研究中的伙伴，是离子通道、G蛋白耦联受体和神经信号工具的主要供应商。我们专业生产基础科学研究中所需的一抗、小分子、蛋白质和多肽。Alomone Labs提供的如下生命科学领域研究方案享誉海内外：离子通道研究领域（一系列全面的离子通道研究产品线，包括标记和未标记的抗体及其相关调节因子）、G蛋白耦联受体研究领域（提供了一整套不断增长的GPCR抗体、受体激动剂和拮抗剂产品名单）、神经信号通路研究（包括一些用于神经营养因子研究的最重要的抗体和生长因子）。 Alomone成立于1989年,已经成为全世界科学家信赖的最全面的离子通道研究工具供应商。所有Alomone产品均进行了生物实验验证，以冻干粉形式提供，可确保产品生物活性和保质期。Alomone可提供多种包装规格，灵活方便。作为Alomone Labs在中国的区域总代理，蚂蚁淘科技有限公司将为中国客户提供最全面的Alomone Labs产品以及客户订制化服务。

公司介绍

品牌分类

腺苷受体附着力等级GPCR 酸敏G蛋白偶联受体肾上腺素能受体

联络我们

服务热线：4000-520-616

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QQ ：1570468124

手机：18915418616

官网：http://www.alomone.com/

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