Alomone/Anti-α2B-Adrenergic Receptor (extracellular) Antibody/AAR-021/0.2 ml-免疫在线蚂蚁淘旗下平台

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商品详细Alomone/Anti-α2B-Adrenergic Receptor (extracellular) Antibody/AAR-021/0.2 ml

Alomone/Anti-α2B-Adrenergic Receptor (extracellular) Antibody/AAR-021/0.2 ml

商品编号： AAR-021-0.2ml

品牌： Alomone Labs

市场价： ¥9500.00

美元价： 5700.00

产地：美国(厂家直采)

公司：

产品分类：受体

公司分类： Receptors

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Cat#:AAR-021

LyophilizedPowder

AntigenIncl.

ShippedatRoomTemp.

Type:Polyclonal

Source:Rabbit

Reactivity:r

Immunogen

Peptide(C)RPEPRGLPQ*SELNQE,correspondingtoaminoacidresidues160-174ofratα_2B-adrenoceptorwithreplacementofcysteine169(C₁₆₉)withserine(*S)(AccessionP19328).2^ndextracellularloop.

Anti-α2B-Adrenergic Receptor (extracellular) Antibody -

Accession(Uniprot)NumberP19328

GeneID24174

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyRat-14/15aminoacidresiduesidentical;mouse-13/15aminoacidresiduesidentical;human-10/14aminoacidresiduesidentical.

PurityAffinitypurifiedonimmobilizedantigen.

FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN₃.

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution25µl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.

Antibodyconcentrationafterreconstitution0.8mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).

ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.

Controlantigenreconstitution100µldoubledistilledwater(DDW).

Controlantigenstorageafterreconstitution-20°C.

PreadsorptionControl1μgpeptideper1μgantibody.

StandardqualitycontrolofeachlotWesternblotanalysis.

Applications:ih,wb

Mayalsoworkin:ic,ifc,ip,lci

Westernblot

Westernblotanalysisofratkidney(lanes1and5),lung(lanes2and6),liver(lanes3and7)lysatesandratskeletalmusclemembranes(lanes4and8):

1.Anti-α_2B-AdrenergicReceptor(extracellular) Antibody(#AAR-021),(1:200).
2.Anti-α_2B-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

Immunohistochemistry

Expressionofα_2B-Adrenoreceptorinratlung

ImmunohistochemicalstainingofratlungparaffinembeddedsectionsusingAnti-α_2B-AdrenergicReceptor(extracellular)Antibody(#AAR-021),(1:100).α_2B-Adrenoreceptorisexpressedintherespiratoryepitheliumofthebronchioli(arrows).Hematoxilinisusedasthecounterstain.

References

IUPHARRECEPTORDATABASE|ADRENOCEPTORS
Piascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.
Link,R.E.etal.(1996)Science273,803.
Makaritsis,K.P.etal.(1999)Hypertension33,14.
Eason,M.G.andLiggett,S.B.(1992)J.Biol.Chem.267,25473.
Pitcher,J.A.etal.(1998)Annu.Rev.Biochem.67,653.
Kurose,H.andLefkowitz,R.J.(1994)J.Biol.Chem.269,10093.

Scientificbackground

Adrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions.

AdrenergicreceptorsaremembersoftheG-proteincoupledreceptor(GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,andacytoplasmiccarboxylterminus.

Adrenoceptorsaredividedintothreetypes:α₁,α₂ andβ-adrenoceptors.Eachtypeisfurtherdividedintoatleastthreesubtypes:α_1A,α_1B,α_1D,α_2A,α_2B,α_2C,β₁,β₂,β₃^1,2.Adrenoceptors areexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem^1,2.

Theα_2B-adrenoceptorhasadistinctpatternofexpressionwithinthebrain,liverlungandkidney,andrecentstudiesusingtheknockoutmousesystemhaveshownthatdisruptionofthisreceptorindeedaffectsmouseviABIlity³,bloodpressureresponsestoα₂-adrenoceptoragoNISTs³ andthehypertensiveresponsetosaltloADIng⁴.

Liketheα_2A-adrenoceptorsubtype,theα_2B-adrenoceptorundergoesshorttermagonistpromoteddesensitization⁵.ThisdesensitizationisduetothephosphorylationofthereceptorbyG-proteincoupledreceptorkinases(GRKs)⁶ whichultimatelypromotesuncouplingofthereceptorfromtheG-proteinsubunit⁷.

LyophilizedPowderControlAntigenIncluded

Clickheretoreceivea25µlfreetrialsample!

Lastupdate:19/02/2019

AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanextracellularepitopeoftheratα_2B-adrenoceptor.Anti-α_2B-AdrenergicReceptor(extracellular)Antibody(#AAR-021)canbeusedinwesternblotandimmunohistochemistryapplications.Ithasbeendesignedtorecognizeα_2B-adrenoceptorfrommouse,rat,andhumansamples.

Forresearchpurposesonly,notforhumanuse

Westernblot

Westernblotanalysisofratkidney(lanes1and5),lung(lanes2and6),liver(lanes3and7)lysatesandratskeletalmusclemembranes(lanes4and8):

1.Anti-α_2B-AdrenergicReceptor(extracellular) Antibody(#AAR-021),(1:200).
2.Anti-α_2B-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

品牌介绍

Alomone Labs是您离子通道研究中的伙伴，是离子通道、G蛋白耦联受体和神经信号工具的主要供应商。我们专业生产基础科学研究中所需的一抗、小分子、蛋白质和多肽。Alomone Labs提供的如下生命科学领域研究方案享誉海内外：离子通道研究领域（一系列全面的离子通道研究产品线，包括标记和未标记的抗体及其相关调节因子）、G蛋白耦联受体研究领域（提供了一整套不断增长的GPCR抗体、受体激动剂和拮抗剂产品名单）、神经信号通路研究（包括一些用于神经营养因子研究的最重要的抗体和生长因子）。 Alomone成立于1989年,已经成为全世界科学家信赖的最全面的离子通道研究工具供应商。所有Alomone产品均进行了生物实验验证，以冻干粉形式提供，可确保产品生物活性和保质期。Alomone可提供多种包装规格，灵活方便。作为Alomone Labs在中国的区域总代理，蚂蚁淘科技有限公司将为中国客户提供最全面的Alomone Labs产品以及客户订制化服务。

公司介绍

品牌分类

腺苷受体附着力等级GPCR 酸敏G蛋白偶联受体肾上腺素能受体

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