阿洛蒙/抗-α；1D肾上腺素能受体（细胞外）抗体/AAR-019/0.2 ml-免疫在线蚂蚁淘旗下平台

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商品详细阿洛蒙/抗-α；1D肾上腺素能受体（细胞外）抗体/AAR-019/0.2 ml

阿洛蒙/抗-α；1D肾上腺素能受体（细胞外）抗体/AAR-019/0.2 ml

商品编号： AAR-019-0.2ml

品牌： Alomone Labs

市场价： ¥9500.00

美元价： 5700.00

产地：美国(厂家直采)

公司：

产品分类：受体

公司分类： Receptors

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Cat#:AAR-019

LyophilizedPowder

AntigenIncl.

ShippedatRoomTemp.

Type:Polyclonal

Source:Rabbit

Reactivity:h,m,r

Immunogen

Peptide(C)EPVPPDERF*SGITEE,correspondingtoaminoacidresidues231-245ofratα_1D-adrenoceptorwithreplacementofcysteine240(C₂₄₀)withserine(*S)(AccessionP23944).3^rdextracellularloop.

Anti-α1D-Adrenergic Receptor (extracellular) Antibody -

Accession(Uniprot)NumberP23944

GeneID29413

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyRat,human,mouse-14/15aminoacidresiduesidentical.

PurityAffinitypurifiedonimmobilizedantigen.

FormulationLyophilizedpowder.Reconstitutedanitbodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN₃.

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.

Antibodyconcentrationafterreconstitution0.8mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).

ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.

Controlantigenreconstitution100µldoubledistilledwater(DDW).

Controlantigenstorageafterreconstitution-20°C.

PreadsorptionControl1μgpeptideper1μgantibody.

StandardqualitycontrolofeachlotWesternblotanalysis.

Applications:ic,ifc,ih,lci,wb

Mayalsoworkin:ip

Westernblot

Westernblotanalysisofratbrainstem(lanes1and3),mousebrain(lanes2and4),SH-SY5Y(lanes5and7)andJurkat(lanes6and8)lysates:

1,2,5,6.Anti-α_1D-AdrenergicReceptor(extracellular) Antibody(#AAR-019),(1:200).
3,4,7,8.Anti-α_1D-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

Immunohistochemistry

Expressionofα_1D-Adrenoceptorinratheart

ImmunohistochemicalstainingofratheartparaffinembeddedsectionsusingAnti-α_1D-AdrenergicReceptor(extracellular)Antibody(#AAR-019),(1:100).α_1D-Adrenoceptorisexpressedincardiomyocytesofthemyocardiumandinthesmoothmuscleofthebloodvessels(arrows).Hematoxilinisusedasthecounterstain.

Expressionofα_1D-Adrenoceptorinratneocortex

Immunohistochemicalstainingofα_1D-adrenoceptorinratneocortexusingAnti-α_1D-AdrenergicReceptor(extracellular)Antibody(#AAR-019). A.Mostintensestainingofα_1D-adrenoceptor(green)appearsinapicaldendrites(thinhorizontalarrows)butalsointhesoma(thickhorizontalarrow).Fewcorticalinterneuronsexpressα_1D-adrenoceptor.B.Thesamesectionwasalsostainedforparvalbuminandonecell(markedwithaverticalarrow)alsoexpressesα_1D-adrenoceptor.

Indirectflowcytometry

IndirectflowcytometryanalysisofintactliveJurkatcells:

^___Unstainedcells.
^___Cells+Anti-α_1D-AdrenergicReceptor(extracellular) Antibody(#AAR-019),(5-10µgantibody/0.5x10⁶cells).

Thecontrolantigenisnotsuitableforthisapplication.

Livecellimaging/Immunocytochemistry

Expressionofα_1D-Adrenoceptor inratPC12cells

Cellsurfacedetectionof α_1D-AdrenoceptorinintactlivingratpheochromocytomaPC12cells.A.ExtracellularstainingofcellsusingAnti-α_1D-AdrenergicReceptor (extracellular) Antibody(#AAR-019)(1:100) followedbygoatanti-rabbit-AlexaFluor-594secondaryantibody.B.MergeofAandliveviewofthecells.

References

IUPHARRECEPTORDATABASE|ADRENOCEPTORS
Piascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.
Chen,Z.J.andMinneman,K.P.(2005)ActaPharmacol.Sin.26,1281.
Tanoue,A.etal.(2002)J.Clin.Invest.109,765.
Chen,Q.etal.(2005)J.Urol.174,370.

Scientificbackground

Adrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions.

AdrenergicreceptorsaremembersoftheG-proteincoupledreceptor(GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,andacytoplasmiccarboxylterminus.

Adrenoceptorsaredividedintothreetypes:α₁,α₂ andβ-adrenoceptors.Eachtypeisfurtherdividedintoatleastthreesubtypes: α_1A, α_1B,α_1D, α_2A, α_2B, α_2C,β₁, β₂, β₃^1,2.Theadrenoceptors areexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem^1,2.

α₁-Adrenergicreceptorsplayanimportantroleinthephysiologicalresponsetoepinephrineandnorepinephrine,particularlyinthecardiovascularsystem.Allthreeclonedα₁ receptors(α_1A,α_1B,andα_1D)coupletoG_q/11.Whiletheircellulardistributionisclear,theirfunctionalroleislesssoduetoalackofspecificagoNISTs/antagonists³.

Asthesereceptorslackspecificagonists/antagonists,knock-out(KO)studiesinmicehaveshedsignificantlightontheircellularrole.Individualα₁-adrenoceptorKOmicedonotdisplayphysicalabnormalitiesingeneral.SimilarKOstudiesindicatethatallα₁ subtypesseemtobeinvolvedintheregulationofbloodpressure³.Also,studiesindicatethatα_1D-adrenoceptorseemstoplayanimportantroleinaorticcontraction⁴,renalfunction,nociception,basallocomotoractivity³ aswellasbladdercontraction⁵.

LyophilizedPowderControlAntigenIncluded

Clickheretoreceivea50µlfreetrialsample!

Lastupdate:19/03/2019

AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanextracellularepitopeoftheratα_1D-adrenergicreceptor.Anti-α_1D-AdrenergicReceptor(extracellular)Antibody(#AAR-019)canbeusedinwesternblot,indirectflowcytometryandimmunohistochemicalapplications.Ithasbeendesignedtorecognizeα_1D-adrenoceptorfrommouse,ratandhumansamples.

Forresearchpurposesonly,notforhumanuse

Westernblot

Westernblotanalysisofratbrainstem(lanes1and3),mousebrain(lanes2and4),SH-SY5Y(lanes5and7)andJurkat(lanes6and8)lysates:

1,2,5,6.Anti-α_1D-AdrenergicReceptor(extracellular) Antibody(#AAR-019),(1:200).
3,4,7,8.Anti-α_1D-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

品牌介绍

Alomone Labs是您离子通道研究中的伙伴，是离子通道、G蛋白耦联受体和神经信号工具的主要供应商。我们专业生产基础科学研究中所需的一抗、小分子、蛋白质和多肽。Alomone Labs提供的如下生命科学领域研究方案享誉海内外：离子通道研究领域（一系列全面的离子通道研究产品线，包括标记和未标记的抗体及其相关调节因子）、G蛋白耦联受体研究领域（提供了一整套不断增长的GPCR抗体、受体激动剂和拮抗剂产品名单）、神经信号通路研究（包括一些用于神经营养因子研究的最重要的抗体和生长因子）。 Alomone成立于1989年,已经成为全世界科学家信赖的最全面的离子通道研究工具供应商。所有Alomone产品均进行了生物实验验证，以冻干粉形式提供，可确保产品生物活性和保质期。Alomone可提供多种包装规格，灵活方便。作为Alomone Labs在中国的区域总代理，蚂蚁淘科技有限公司将为中国客户提供最全面的Alomone Labs产品以及客户订制化服务。

公司介绍

品牌分类

腺苷受体附着力等级GPCR 酸敏G蛋白偶联受体肾上腺素能受体

联络我们

服务热线：4000-520-616

（限工作日9:00-18:00）

QQ ：1570468124

手机：18915418616

官网：http://www.alomone.com/

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