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当前位置: 首页 > 产品中心 > Receptors > 阿洛蒙/抗-alpha;1B肾上腺素能受体(细胞外)-ATTO-488抗体/AAR-018-AG/50µl
商品详细阿洛蒙/抗-alpha;1B肾上腺素能受体(细胞外)-ATTO-488抗体/AAR-018-AG/50µl
阿洛蒙/抗-alpha;1B肾上腺素能受体(细胞外)-ATTO-488抗体/AAR-018-AG/50µl
阿洛蒙/抗-alpha;1B肾上腺素能受体(细胞外)-ATTO-488抗体/AAR-018-AG/50µl
商品编号: AAR-018-AG-50µl
品牌: Alomone Labs
市场价: ¥10540.00
美元价: 6324.00
产地: 美国(厂家直采)
公司:
产品分类: 受体
公司分类: Receptors
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Cat#:AAR-018-AG
  • LyophilizedPowder
  • AntigenIncl.
  • ShippedatRoomTemp.
  • Type:Polyclonal
    Source:Rabbit
    Reactivity:h,r
    Immunogen
    Peptide(C)KNANFTGPNQTSSNS,correspondingtoaminoacidresidues21-35ofhumanα1B-adrenoceptor(Accession P35368).Extracellular,N-terminus.
    Accession(Uniprot)NumberP35368
    GeneID147
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyMouse-14/15aminoacidresiduesidentical;rat-13/15aminoacidresiduesidentical.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    LabelATTO-488.Maximumabsorption501nm;maximumfluorescence523nm.Thefluorescenceisexcitedmostefficientlyinthe480–515nmrange.Thislabelisanalogoustothewellknowndyefluoresceinisothiocyanate(FITC)andcanbeusedwithfilterstypicallyusedtodetectFITC.
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution50µldoubledistilledwater(DDW).
    Antibodyconcentrationafterreconstitution1mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).
    ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
    Controlantigenreconstitution100µldoubledistilledwater(DDW).
    Controlantigenstorageafterreconstitution-20°C.
    PreadsorptionControl1μgpeptideper1μgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#AAR-018),andlivecellimaging(labeledantibody).
    Applications:fc,ic,lci
    Mayalsoworkin:ih
    Directflowcytometry
    DirectflowcytometryanalysisinratGH3pituitaryadenomacells:
    ___Cells.
    ___Cells+ Anti-α1B-AdrenergicReceptor(extracellular)-ATTO-488 Antibody(#AAR-018-AG),(10µg/5x10cells).
    Livecellimaging/Immunocytochemistry
    Expressionofα1B-AdrenoceptorinratGH3cells
    ImmunocytochemicalstainingofGH3cellswithAnti-α1B-AdrenergicReceptor(extracellular)-ATTO-488Antibody (#AAR-018-AG)(green).Nuclearfluorescencestainingofcellsusingthemembrane-permeableDNAdyeHoechst33342(blue).
    References
    1. IUPHARRECEPTORDATABASE|ADRENOCEPTORS
    2. Piascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.
    3. Perez,D.M.etal.(1993)Mol.Pharmacol.44,784.
    4. Minneman,K.P.(1988)Pharmacol.Rev.40,87.
    5. Schwinn,D.A.etal.(2004)MayoClin.Proc.79,1423.
    Scientificbackground

    Adrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions.

     
    Adrenergicreceptorsaremembersofthe G-proteincoupledreceptor (GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,andacytoplasmiccarboxylterminus.

    Adrenoceptorsaredividedintothreetypes:α12 andβadrenoceptors.Eachtypeisfurtherdividedintoatleastthreesubtypes:α1A1B1D2A2B2C123.1,2 Theyareexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem.1,2
     
    Allα1-adrenoceptors(α1-ARs)activatephospholipasesCandA2.3 Inadditiontomobilizingintracellularcalcium,theα1-ARshavealsobeenshowntoactivatecalciuminfluxviavoltage-dependentand-independentcalciumchannels.4
     
    α1B-Adrenoceptorpopulationsaregreatestinthespleen,kidney,cerebellum,andfetalbrain.5

    α1B-Adrenoceptorcausescontractionofsmoothmusclecellsandtherebycontrolsvasculartone,bloodpressure,andacceleratesthedevelopmentofatherosclerosis.5

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    Lastupdate:24/03/2019

    Anti-α1BAdrenergicReceptor(extracellular)Antibody(#AAR-018)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthehumanα1B-adrenoceptor.Theantibodycanbeusedinwesternblot,immunohistochemistry,livecellimagingandindirectflowcytometryapplications.Ithasbeendesignedtorecognizeα1B-adrenoceptorfromhuman,ratandmousesamples.

    Anti-α1B-AdrenergicReceptor(extracellular)-ATTO-488Antibody(#AAR-018-AG)isdirectlylabeledwithanATTO-488fluorescentdye.ATTOdyesarecharacterizedbystrongabsorption(highextinctioncoefficient),highfluorescencequantumyield,andhighphoto-stABIlity.TheATTO-488labelisanalogoustothewellknowndyefluoresceinisothiocyanate(FITC)andcanbeusedwithfilterstypicallyusedtodetectFITC.Anti-α1B-AdrenergicReceptor(extracellular)-ATTO-488Antibody hasbeentestedindirectflowcytometryapplicationsandisspeciallysuitedtoexperimentsrequiringsimultaneouslabelingofdifferentMarkers.

    Forresearchpurposesonly,notforhumanuse
    Directflowcytometry
    DirectflowcytometryanalysisinratGH3pituitaryadenomacells:
    ___Cells.
    ___Cells+ Anti-α1B-AdrenergicReceptor(extracellular)-ATTO-488 Antibody(#AAR-018-AG),(10µg/5x10cells).
    Livecellimaging/Immunocytochemistry
    Expressionofα1B-AdrenoceptorinratGH3cells
    ImmunocytochemicalstainingofGH3cellswithAnti-α1B-AdrenergicReceptor(extracellular)-ATTO-488Antibody (#AAR-018-AG)(green).Nuclearfluorescencestainingofcellsusingthemembrane-permeableDNAdyeHoechst33342(blue).
    References
    1. IUPHARRECEPTORDATABASE|ADRENOCEPTORS
    2. Piascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.
    3. Perez,D.M.etal.(1993)Mol.Pharmacol.44,784.
    4. Minneman,K.P.(1988)Pharmacol.Rev.40,87.
    5. Schwinn,D.A.etal.(2004)MayoClin.Proc.79,1423.
    品牌介绍
           Alomone Labs是您离子通道研究中的伙伴,是离子通道、G蛋白耦联受体和神经信号工具的主要供应商。我们专业生产基础科学研究中所需的一抗、小分子、蛋白质和多肽。Alomone Labs提供的如下生命科学领域研究方案享誉海内外:离子通道研究领域(一系列全面的离子通道研究产品线,包括标记和未标记的抗体及其相关调节因子)、G蛋白耦联受体研究领域(提供了一整套不断增长的GPCR抗体、受体激动剂和拮抗剂产品名单)、 神经信号通路研究(包括一些用于神经营养因子研究的最重要的抗体和生长因子)。          Alomone成立于1989年,已经成为全世界科学家信赖的最全面的离子通道研究工具供应商。所有Alomone产品均进行了生物实验验证,以冻干粉形式提供,可确保产品生物活性和保质期。Alomone可提供多种包装规格,灵活方便。作为Alomone Labs在中国的区域总代理,蚂蚁淘科技有限公司将为中国客户提供最全面的Alomone Labs产品以及客户订制化服务。