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当前位置: 首页 > 产品中心 > Receptors > 阿洛蒙/抗EMR1(细胞外)-FITC抗体/AER-051-F/5 x 50µl
商品详细阿洛蒙/抗EMR1(细胞外)-FITC抗体/AER-051-F/5 x 50µl
阿洛蒙/抗EMR1(细胞外)-FITC抗体/AER-051-F/5 x 50µl
阿洛蒙/抗EMR1(细胞外)-FITC抗体/AER-051-F/5 x 50µl
商品编号: AER-051-F-50µl
品牌: Alomone Labs
市场价: ¥10540.00
美元价: 6324.00
产地: 美国(厂家直采)
公司:
产品分类: 受体
公司分类: Receptors
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Cat#:AER-051-F
  • LyophilizedPowder
  • AntigenIncl.
  • ShippedatRoomTemp.
  • Type:Polyclonal
    Source:Rabbit
    Reactivity:h,m,r
    Immunogen
    PeptideCKQGFLSSNGQNHFK,correspondingtoaminoacidresidues58-72ofhumanEMR1(Accession Q14246).Extracellular,N-terminus.
    Accession(Uniprot)NumberQ14246
    GeneID2015
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyMouse,Rat–11/14aminoacidresiduesidentical.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    LabelFluoresceinisothiocyanate(FITC).
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution50µldoubledistilledwater(DDW).
    Antibodyconcentrationafterreconstitution1mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).
    ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefortwoweeks.Forlongerperiods,itshouldbestoredat-20°C.
    Controlantigenreconstitution100µldoubledistilledwater(DDW).
    Controlantigenstorageafterreconstitution-20°C.
    PreadsorptionControl1µgpeptideper1µgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#AER-051),anddirectflowcytometry(labeledantibody).
    Applications:fc
    Mayalsoworkin:ic,lci
    Directflowcytometry
    DirectflowcytometryofliveintactmouseJ774macrophagecells:
    ___ Cells.
    ___ Cells+rabbitIgGisotypecontrol-FITC.
    ___ Cells+Anti-EMR1(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).
    DirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:
    ___ Cells.
    ___ Cells+rabbitIgGisotypecontrol-FITC.
    ___ Cells+Anti-EMR1(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).
    References
    1. Legrand,F.etal.(2014)J.AllergyClin.Immunol.133,1439.
    2. McKnight,A.J.andGordon,S.(1998)J.Leukoc.Biol.63,271.
    3. Araç,D.etal.(2012)EMBOJ.31,1364.
    Scientificbackground

    Humanepidermalgrowthfactor(EGF)–likemodulecontainingmucin-likehormonereceptor1(EMR1)isasurfacereceptorwithunknownfunctionthatbelongstotheadhesionfamilyoftheGprotein-coupledreceptors(GPCR).ThisreceptorisencodedbytheADGRE1andlikeallGPCRs,containsseventransmembranedomains,anintracellularC-terminusandanextracellularN-terminus.TheNH2domainhassixEGF-likedomains.

    AdhesionGPCRssharealongN-terminalextracellularsequencescomprisingmultipledomains,separatedfromthetransmembranesegmentsbyaserine/threonine-richdomain,afeaturereminiscentofmucin-like,single-span,integralmembraneglycoproteinswithadhesiveproperties1,2.

    EMR1proteinisahomologofF4/80,amurineproteinthatiswidelybeingusedasaMarkerofmurinemacrophagepopulations1.

    HumanEMR1isexclusivelyexpressedonmatureeosinophilsinthebloodandbonemarrow andinnasalpolyps.Thus,EMR1is consideredtobeapotentialtherapeutictargetforthetreatmentofeosinophilicdisorders1,3.

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat

    Checkoutmoreofournewproducts.

    Lastupdate:20/03/2019

    Anti-EMR1(extracellular)Antibody (#AER-051)isahighlyspecificantibodydirectedagainstanepitopeofthehumanprotein.Theantibodycanbeusedinwesternblotandlivecellimagingapplications.Itrecognizesanextracellularepitopeandisthusidealfordetectingthereceptorinlivingcells.IthasbeendesignedtorecognizeEMR1fromhuman,mouse,andratsamples.

    Anti-EMR1(extracellular)FITCAntibody (#AER-051-F)is directlyconjugatedtofluoresceinisothiocyanate(FITC). The antibodycanbeusedinimmunofluorescentapplicationssuchasdirectlivecellflowcytometry.

    Forresearchpurposesonly,notforhumanuse
    Directflowcytometry
    DirectflowcytometryofliveintactmouseJ774macrophagecells:
    ___ Cells.
    ___ Cells+rabbitIgGisotypecontrol-FITC.
    ___ Cells+Anti-EMR1(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).
    DirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:
    ___ Cells.
    ___ Cells+rabbitIgGisotypecontrol-FITC.
    ___ Cells+Anti-EMR1(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).
    References
    1. Legrand,F.etal.(2014)J.AllergyClin.Immunol.133,1439.
    2. McKnight,A.J.andGordon,S.(1998)J.Leukoc.Biol.63,271.
    3. Araç,D.etal.(2012)EMBOJ.31,1364.
    品牌介绍
           Alomone Labs是您离子通道研究中的伙伴,是离子通道、G蛋白耦联受体和神经信号工具的主要供应商。我们专业生产基础科学研究中所需的一抗、小分子、蛋白质和多肽。Alomone Labs提供的如下生命科学领域研究方案享誉海内外:离子通道研究领域(一系列全面的离子通道研究产品线,包括标记和未标记的抗体及其相关调节因子)、G蛋白耦联受体研究领域(提供了一整套不断增长的GPCR抗体、受体激动剂和拮抗剂产品名单)、 神经信号通路研究(包括一些用于神经营养因子研究的最重要的抗体和生长因子)。          Alomone成立于1989年,已经成为全世界科学家信赖的最全面的离子通道研究工具供应商。所有Alomone产品均进行了生物实验验证,以冻干粉形式提供,可确保产品生物活性和保质期。Alomone可提供多种包装规格,灵活方便。作为Alomone Labs在中国的区域总代理,蚂蚁淘科技有限公司将为中国客户提供最全面的Alomone Labs产品以及客户订制化服务。